serine/arginine-Rich Splicing Factor 2 (SRSF2) antibody

Antigen: serine/arginine-Rich Splicing Factor 2 (SRSF2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Western Blotting (WB)

Catalog no.: ABIN123803

Additional Information

Alternative name: Splicing Component, 35 kDa

Format: Liquid

Description: SC35, a member of SR protein family, is an essential pre-mRNA splicing factor and can function as an essential splicing factor as well as an alternative splicing factor. Phosphorylation on serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly interacts with the C-terminal domain (CTD) of the largest subunit of RNA Polymerase II, thereby suggesting a potential role of SC35 in coordinating of transcription and pre-mRNA splicing. alpha-SC35 is an affinity-purified rabbit polyclonal antibody raised against the recombinant SC35 protein.

Specificity: Alpha-SC35 reacts with the SC35 protein in HeLa nuclear extract by Western blotting.

Application Details

Application Notes: Recommended dilution range for Western blot analysis: 1:200 - 1:1000.

Storage: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Restrictions: For Research Use only

Publications

Fu, Maniatis: "Isolation of a complementary DNA that encodes the mammalian splicing factor SC35." in: Science (New York, N.Y.), Vol. 256, Issue 5056, pp. 535-8, 1992 (PubMed).

Zahler, Lane, Stolk et al.: "SR proteins: a conserved family of pre-mRNA splicing factors." in: Genes & development, Vol. 6, Issue 5, pp. 837-47, 1992 (PubMed).

Gui, Tronchere, Chandler et al.: "Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 23, pp. 10824-8, 1994 (PubMed).

Colwill, Pawson, Andrews et al.: "The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution." in: The EMBO journal, Vol. 15, Issue 2, pp. 265-75, 1996 (PubMed).

Mortillaro, Blencowe, Wei et al.: "A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8253-7, 1996 (PubMed).

Misteli, Caceres, Spector: "The dynamics of a pre-mRNA splicing factor in living cells." in: Nature, Vol. 387, Issue 6632, pp. 523-7, 1997 (PubMed).