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Actin antibody
| Antigen | Actin |
| Synonyms |
A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G ...
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| Clonality | Monoclonal (HHF35) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. | ABIN125105 |
| Quantity | 500ul (100ug/ml approximately) (Variants) |
| Price | 197.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business days |
Additional Information
| Immunogen | BALB/C mice were injected with SDS extracted protein from human myocardium. |
| Format | Supernatant |
| Isotype | IgG1-kappa (Matching secondary antibodies) |
| Clone | HHF35 |
| Specificity | This antibody is specific to alpha and gamma specific actin isomers from skeletal, cardiac and smooth muscle but does not recognize beta and nonsmooth muscle gamma actin isomers. In Western blot, HHF35 picks up 42 kD protein extract from skeletal muscle but not from epithelial cell line. |
| Synonyms | A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051 |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as tissue culture supernatant containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Alternatives
Alternatives for antigen "Actin", type "Antibodies"
| Hosts | Mouse (58), Rabbit (18), Sheep (3) |
| Reactivities | Human (46), Chicken (23), Rat (Rattus) (20), Mouse (Murine) (12), Cow (Bovine) (11), Rabbit (10), Snake (8), Carp (6), Frog (6), Guinea Pig (6), Sheep (Ovine) (5), All Species (3), Dog (Canine) (3), Cat (Feline) (2), Pig (Porcine) (2), Xenopus laevis (2), Dictyostelium (1), Horse (Equine) (1), Physarum (1), Primate (1), Primates (1) |
| Applications | Western Blotting (WB) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunofluorescence (IF) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), ELISA (16), Immunohistochemistry (IHC) (16), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (7), Enzyme Immunoassay (EIA) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (6), Cy3 (1), FITC (1) |
| Epitopes | C-Term (6), pan (6), N-Term (3), Ab-55/53 (1), Asp244 (1), pTyr55/53 (1) |
