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Antibodies
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anti-Carcinoembryonic Antigen (CEA) antibody
Carcinoembryonic Antigen (CEA) antibody
| Antigen |
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| Synonyms |
CEACAM5, CEA, CEACAM, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik |
| Clonality |
Monoclonal (Col-1) |
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Host
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Reactivity
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Human (92), Mouse (Murine) (15), Rat (Rattus) (15), Cow (Bovine) (10), Dog (Canine) (9), Pig (Porcine) (5), Horse (Equine) (3), Chicken (2), Rabbit (2), Cat (Feline) (1), Goat (1), Lizard (1), Zebrafish (Brachydanio rerio) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (32), Immunohistochemistry (IHC) (31), Immunofluorescence (IF) (28), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Western Blotting (WB) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (7), Enzyme Immunoassay (EIA) (6), Radioimmunoassay (RIA) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), To be determined by user (TBD) (1)
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| Catalog no. |
ABIN125109 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
137.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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BALB/C mice were injected with extract of colon carcinoma cells.
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Format
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Supernatant
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Isotype
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IgG2a kappa
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Clone
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Col-1
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Specificity
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This antibody is a member of family of complex antigens, which also includes non-specific cross-reacting antigens (NCA). This antibody stains specific regions of CEA. It reacts with colorectal adenocarcinomas but does not show any staining with polymorphonuclear neutrophils and erythrocytes.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:80 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues may be required before immunostaining. Positive Control: Colan Carcinoma. Cellular localization: Cytoplasmic and lumenal membrane Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as immunoglobulin fraction from mouse ascites fluid containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Carcinoembryonic Antigen (CEA)", type "Antibodies"
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Hosts
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Mouse (73), Rabbit (38), Goat (7)
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Reactivities
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Human (92), Mouse (Murine) (15), Rat (Rattus) (15), Cow (Bovine) (10), Dog (Canine) (9), Pig (Porcine) (5), Horse (Equine) (3), Chicken (2), Rabbit (2), Cat (Feline) (1), Goat (1), Lizard (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Flow Cytometry (FACS) (32), Immunohistochemistry (IHC) (31), Immunofluorescence (IF) (28), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Western Blotting (WB) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (7), Enzyme Immunoassay (EIA) (6), Radioimmunoassay (RIA) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), To be determined by user (TBD) (1)
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Conjugates
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HRP (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), PE,Cy5.5 (3), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Center (1)
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