Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8)

Synonyms: CD30, Ki-1, D1S166E, Ki, Cd30, RP23-306F22.1, TNFRSF8, TNFSF8

Clonality: Monoclonal (Ber-H2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125112

Additional Information

Alternative name: CD30

Immunogen: BALB/C mice were immunized with L428 cell line.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: Ber-H2

Specificity: This antibody reacts with a 105 kD glycoprotein expressed by Hodgkin's and Reed-Sternberg cells and by tumors cells of majority of large cell lymphomas. In normal lymphoid tissues, it reacts with a small population of large cells present around B cell follicles and some times at the rim of germinal centers.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human Hodgkins lymphoma stained with CD30 antibody