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Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody

Antigen

Platelet/endothelial Cell Adhesion Molecule (PECAM1)
Synonyms CD31, PECAM-1, FLJ58394, Cd31, Pecam, C85791, MGC102160, MGC124998, PECAM1
Clonality Monoclonal (JC-70A)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125114
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 177.00 $   Plus shipping costs $35.00
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name CD31
Immunogen BALB/C mice were immunized with membrane preparation of a spleen from a patient with hairy cell leukemia.
Format Supernatant
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone JC-70A
Specificity This antibody reacts with a 100 kD glycoprotein expressed by endothelial cells and 130 kD glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues.

Application Details
Images Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic digestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Platelet/endothelial Cell Adhesion Molecule (PECAM1)", type "Antibodies"
Hosts Mouse (125), Rabbit (61), Rat (17), Hamster (2), Sheep (1)
Reactivities Human (156), Mouse (Murine) (69), Rat (Rattus) (54), Dog (Canine) (35), Pig (Porcine) (33), Horse (Equine) (18), Rabbit (17), Cow (Bovine) (7), Sheep (Ovine) (6), Monkey (5), Chicken (2)
Applications Flow Cytometry (FACS) (150), Western Blotting (WB) (67), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunohistochemistry (IHC) (22), Immunoprecipitation (IP) (22), ELISA (16), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), ELISA (Detection) (3), Functional Studies (Func) (3), Blocking Antibody (Inhibition) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (21), Biotin (14), PE (13), APC (6), PE,Cy7 (4), Alexa Fluor 488 (3), RPE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), RBITC (2), Alexa Fluor 700 (1), PE,DY590 (1), PE-Dyomics 590 (1), Pacific Blue (1), PerCP (1)
Epitopes C-Term (13), pTyr713 (1)