Cytomegalovirus, Coctail antibody

Antigen: Cytomegalovirus, Coctail

Clonality: Monoclonal (DDG9-CCH2)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125122

Additional Information

Alternative name: Cytomegalovirus (CMV) Cocktail

Immunogen: BALB/C mice were immunized with cytomegalovirus infected cell lysate.

Format: Supernatant

Isotype: IgG1, IgG2a kappa  (Matching secondary antibodies)

Clone: DDG9-CCH2

Specificity: This cocktail antibody reacts with an immediate early and early CMV antigens. The antibody stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen. In Western blot, DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Infected lung. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is a cocktail of two monoclonal antibodies against CMV and is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human salivary glands stained with CMV antibody