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Antibodies
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anti-Cytokeratin 18 (KRT18) antibody
Cytokeratin 18 (KRT18) antibody
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Clonality |
Monoclonal (CY-90) |
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Host
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Reactivity
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Human (124), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (9), Hamster (5), None (4), Sheep (Ovine) (3), Cat (Feline) (2), Bird (Avian) (1), Monkey (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (64), Flow Cytometry (FACS) (63), Immunofluorescence (IF) (58), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), ELISA (20), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (14), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN125126 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
197.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin 18
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Immunogen
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Human epidermal carcinoma and MCF-7 human breast cancer cells.
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Format
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Ascites
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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CY-90
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Specificity
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This antibody recognizes keratin polypeptide of 45 kD. In normal tissues, it reacts with a large number of epithelial cell types, including ductal and glandular epithelia. This antibody does not react with squamous epithelia. Most of the adenocarcinomas, basal cell carcinomas and benign and malignant epithelial lesions stain with this antibody.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic digestion may be required in some paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as diluted ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Rabbit (75), Mouse (70), Guinea Pig (1)
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Reactivities
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Human (124), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (9), Hamster (5), None (4), Sheep (Ovine) (3), Cat (Feline) (2), Bird (Avian) (1), Monkey (1)
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Applications
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Western Blotting (WB) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (64), Flow Cytometry (FACS) (63), Immunofluorescence (IF) (58), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), ELISA (20), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (14), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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FITC (13), Biotin (11), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitopes
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pSer33 (7), C-Term (5), Center (1), N-Term (1)
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