Keratin High Molecular Weight antibody

Antigen: Keratin High Molecular Weight

Clonality: Monoclonal (34bE12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125133

Additional Information

Alternative name: Cytokeratin (H.M.Weight)

Immunogen: BALB/C mice were immunized with solublized keratin extract from human stratum corneum.

Clone: 34bE12

Specificity: This antibody recognizes keratin polypeptide of 68, 58, 56.5 and 50 kD in extract of stratum corneum. The antibody reacts with squamous, ductal and other complex epithelia. It stains with adenocarcinomas, breast, pancreas, bile duct, salivary gland and transitional cell carcinomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human skin stained with Cytokeratin, H.M.W. antibody