Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Glial Fibrillary Acidic Protein (GFAP) antibody

Antigen

Glial Fibrillary Acidic Protein (GFAP)
Synonyms GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734
Clonality Monoclonal (GA5)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125137
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 170.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 5 Business Days

anti-Glial Fibrillary Acidic Protein (GFAP) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Immunogen BALB/C mice were immunized with glial fibrillary protein from porcine spinal cord.
Format Supernatant
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone GA5
Specificity This antibody reacts with the 52 kD intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins.

Application Details
Images Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasm Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cell/Tissue Markers, Cytoskeleton, Neurology
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Glial Fibrillary Acidic Protein (GFAP)", type "Antibodies"
Hosts Mouse (150), Rabbit (82), Chicken (12), Goat (4), Guinea Pig (2)
Reactivities Human (164), Rat (Rattus) (69), Pig (Porcine) (66), Mouse (Murine) (51), Cow (Bovine) (46), Sheep (Ovine) (21), Rabbit (19), Dog (Canine) (18), Chicken (13), Guinea Pig (12), Mammalian (11), Cat (Feline) (8), Goat (3), Bird (Avian) (2), Caprinae (2), Hamster (1), Horse (Equine) (1), Monkey (1), Primate (1)
Applications Western Blotting (WB) (152), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (106), Immunofluorescence (IF) (99), Immunohistochemistry (Frozen Sections) (IHC (fro)) (62), Immunohistochemistry (IHC) (46), Immunoprecipitation (IP) (31), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Capture) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), Cy3 (3), HRP (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (1), internal (1), pSer8 (1)