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Mouse anti-Human Immunoglobulin Kappa Antibody

Antigen

Immunoglobulin Kappa

Clonality Monoclonal (KP-53)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Immunohistochemistry (IHC)
Catalog no. ABIN125145
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 114.00 $   Plus shipping costs $35.00
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Additional Information

Immunogen BALB/C mice were immunized with purified Bence Jones kappa light chain protein.
Format Supernatant
Isotype IgG2b kappa  (Matching secondary antibodies)
Clone KP-53
Specificity This antibody reacts with B cell follicles in human lymphoid tissues. The mantle zones give a mosaic pattern, while the germinal centers show a coarse meshwork pattern of staining. This antibody stains free as well as bound kappa light chains. This antibody does not react with human lambda light chain.

Application Details

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Although not required, enzymatic predigestion may help in some cases. Positive Control: Tonsil. Cellular localization: Cytoplasm
Concentration 100ug/ml approximately
Buffer This antibody is supplied as diluted ascites containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives

Alternatives for antigen "Immunoglobulin Kappa", type "Secondary Antibodies"
Hosts Goat (7), Mouse (2), Rabbit (2)
Reactivities Human (11)
Conjugates Alkaline Phosphatase (AP) (1), Biotin (1), FITC (1), Peroxidase (POD) (1), Rhodamine (1), Texas Red (TR) (1)