Neurofilament L&H (70/200 KD) antibody

Antigen: Neurofilament L&H (70/200 KD)

Clonality: Monoclonal (2F11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125148

Additional Information

Immunogen: BALB/C mice were injected with purified neurofilaments from human brain.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 2F11

Specificity: This antibody reacts with 70 and 200 kD component of the polypeptide subunit. It stains neurons, neuronal processes and peripheral nerves. This antibody reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytoskeleton, Cell/Tissue Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human cerebellum stained with Neurofilament antibody

Publications

Publications: Marton, Feletti, Basaldella et al.: "Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with literature review." in: Acta paediatrica (Oslo, Norway : 1992), Vol. 97, Issue 8, pp. 1145-9, 2008 (PubMed).