Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Monoclonal (DO-7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125150
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 244.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 5 Business Days

anti-Tumor Protein P53 (TP53) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name p53
Immunogen Recombinant human wild type P53 protein expressed in E. coli.
Format Supernatant
Isotype IgG2b kappa  (Matching secondary antibodies)
Clone DO-7
Specificity This antibody reacts with wild as well as mutant types of P53 protein. It recognizes an epitope in the N-terminus of P53 protein, known to reside between amino acids 35 to 45. This antibody can be demonstrated in 22-76% of the colon, stomach, bladder, breast, lung and testes cancers.

Application Details
Images Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast Carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Transcription Factors
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (255), Mouse (79), Sheep (3)
Reactivities Human (328), Mouse (Murine) (68), Rat (Rattus) (62), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Horse (Equine) (3), Dog (Canine) (2), Hamster (2), Primate (2), Primates (2), Sheep (Ovine) (2), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (279), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (85), Immunohistochemistry (IHC) (81), ELISA (68), Immunofluorescence (IF) (61), Immunoprecipitation (IP) (41), Enzyme Immunoassay (EIA) (40), Flow Cytometry (FACS) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (8), Transfected cell culture (TCC) (3), Radioimmunoassay (RIA) (2), Chromatin Immunoprecipitation (ChiP) (1)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer37 (11), pSer315 (10), pSer33 (9), pSer46 (9), pThr18 (9), pSer392 (8), pSer6 (8), pSer15 (7), AA 371-380 (5), pSer20 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer9 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), pSer376 (1), pSer378 (1), pThr55 (1), phospho (1)