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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal (V9)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125156
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 179.00 $   Plus shipping costs $35.00
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anti-Vimentin (VIM) antibody

Additional Information
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Alternative name Vimentin
Immunogen BALB/C mice were injected with purified vimentin from porcine eye lens.
Format Supernatant
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone V9
Specificity This antibody reacts with the 57 kD intermediate filament protein present in the cells of mesenchymal origin. The antibody does not show any cross-reactivity with other intermediate filament proteins. However, it shows a broad interspecies cross- reactivity.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Sarcoma. Cellular localization: Cytoplasm Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin from the ascites by Protein G chromatography and contains sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cell/Tissue Markers, Cytoskeleton
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Rabbit (89), Mouse (63), Chicken (8), Sheep (3), Guinea Pig (2), Goat (1)
Reactivities Human (144), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (31), Cow (Bovine) (26), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (90), Immunofluorescence (IF) (68), Immunohistochemistry (IHC) (55), Flow Cytometry (FACS) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (13), Immunoprecipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2), Dot Blot (Dot) (1)
Conjugates FITC (9), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitopes pSer82 (20), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)