CD68 (CD68) antibody
| Antigen |
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| Synonyms |
GP110, SCARD1, DKFZp686M18236 |
| Clonality |
Monoclonal (PG-M1) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunofluorescence (IF) (29), Western Blotting (WB) (28), Immunoprecipitation (IP) (17), ELISA (4), Immunohistochemistry (IHC) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125158 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
215.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD68
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Immunogen
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BALB/C mice were injected with Gaucher cells.
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Format
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Supernatant
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Isotype
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IgG3 kappa (Matching secondary antibodies)
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Clone
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PG-M1
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Specificity
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This antibody reacts with a glycoprotein of 110 kD, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. The PG-M1 differs from EMB11 (CD68) because of its non-reactivity with granulocytes and their precursor cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:40 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. PG-M1 is not recommended for frozen sections or cell smears. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD68 (CD68)", type "Antibodies"
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Hosts
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Mouse (69), Rabbit (33), Rat (23)
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Reactivities
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Human (72), Mouse (Murine) (43), Rat (Rattus) (34), Cow (Bovine) (7), Monkey (2), Cat (Feline) (1), Dog (Canine) (1), Guinea Pig (1), Pig (Porcine) (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Flow Cytometry (FACS) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunofluorescence (IF) (29), Western Blotting (WB) (28), Immunoprecipitation (IP) (17), ELISA (4), Immunohistochemistry (IHC) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (16), Biotin (8), PE (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Epitopes
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AA 155-355 (3), AA 1-164 (1)
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