Deoxynucleotidyltransferase, terminal (DNTT) antibody

Antigen: Deoxynucleotidyltransferase, terminal (DNTT)

Synonyms: TDT, Tdt, BB160593, DNTT, MGC109152, dntt-A, TdT

Clonality: Monoclonal (6A6-09)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125159

Additional Information

Alternative name: TdT

Immunogen: BALB/C mice were injected with Bovine TdT

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 6A6-09

Specificity: This antibody stains a 60kD peptide in human lymphoblasts. TdT is present in the cells during immunoglobulin gene rearrangement and plays an important role in lymphocytes maturation. TdT marks both T and B immature lymphocytes normally found in thymus and bone marrow.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Paraffin-embedded tissue sections require high temperature antigen unmasking using 10 mM citrate buffer, pH 6.0. Positive Control: Thymus. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union