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CD3 (CD3E) antibody

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (PS1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125166
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 233.00 $   Plus shipping costs $35.00
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anti-CD3 (CD3E) antibody

Additional Information
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Alternative name CD3
Immunogen Epsilon Chain of CD3.
Format Ig-fraction
Isotype IgG2a  (Matching secondary antibodies)
Clone PS1
Specificity This antibody reacts specifically with the epsilon chain of the CD3 complex. This antibody is a pan T cell marker. On the lymphocyte surface, this antibody binds to 22/26/30 kD molecule, which is associated with T cell antigen receptor complex. This antibody reacts with T cell chronic lymphocytic leukemias as well as with acute lymphoblastic leukemias of T cell origin.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (302), Rat (53), Rabbit (49), Hamster (35)
Reactivities Human (284), Mouse (Murine) (100), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (18), Cow (Bovine) (17), Monkey (15), Primate (10), Bird (Avian) (4), Cat (Feline) (4), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (347), Immunohistochemistry (Frozen Sections) (IHC (fro)) (86), Immunoprecipitation (IP) (61), Functional Studies (Func) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Western Blotting (WB) (36), Immunofluorescence (IF) (31), ELISA (19), Immunohistochemistry (IHC) (17), Blocking Antibody (Inhibition) (10), Immunocytochemistry (ICC) (6), Enzyme Immunoassay (EIA) (3), Cytotoxicity Test (CyTox) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (46), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)