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CD8a Molecule (CD8A) antibody

Antigen

CD8a Molecule (CD8A)
Synonyms CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Clonality Monoclonal (144B)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125167
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 386.00 $   Plus shipping costs $35.00
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anti-CD8a Molecule (CD8A) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name CD8
Immunogen Synthetic peptide comprising of 13 C terminal amino acids of the cytoplasmic domain of CD8 molecule.
Format Supernatant
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone 144B
Specificity This antibody reacts specifically with a 32 kD protein similar to CD8 molecule. This antibody stains cytotoxic/suppressor T cells and shows a staining patterns similar to clustered CD8 monoclonal antibodies.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"
Hosts Mouse (299), Rat (103), Rabbit (77)
Reactivities Human (275), Mouse (Murine) (117), Rat (Rattus) (51), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Applications Flow Cytometry (FACS) (380), Immunohistochemistry (Frozen Sections) (IHC (fro)) (125), Immunoprecipitation (IP) (73), Immunofluorescence (IF) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Western Blotting (WB) (37), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (1)
Conjugates FITC (84), Biotin (47), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), PerCP (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitopes C-Term (1), N-Term (1)