CD79a Molecule, Immunoglobulin-Associated alpha (CD79A) antibody

Antigen: CD79a Molecule, Immunoglobulin-Associated alpha (CD79A)

Synonyms: IGA, MB-1, CD79A, IG-alpha

Clonality: Monoclonal (HM57)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125168

Additional Information

Alternative name: CD79a

Immunogen: Synthetic peptide comprising of 202-216 C terminal amino acids part of mb-1 protein.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: HM57

Specificity: This antibody reacts specifically with B cells at the pre B cell stage. It stains majority of acute leukemias of precursor B cell type, B cell lymphomas and some myelomas. The mb-1 polypeptide along with B29 polypeptide forms an antigen receptor complex on the B cell performing signal transaction and phosphorylation functions.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Old, Carman, Fry et al.: "The immune tissues of the endangered red-tailed phascogale (Phascogale calura)." in: Journal of anatomy, Vol. 208, Issue 3, pp. 381-7, 2006 (PubMed).