Carbohydrate Antigen 15-3 (CA15-3) antibody

Antigen: Carbohydrate Antigen 15-3 (CA15-3)

Synonyms: MUC1

Clonality: Monoclonal (DF3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125179

Additional Information

Alternative name: CA 15-3

Immunogen: BALB/C mice were injected with membrane rich fraction of human metastatic breast carcinoma.

Format: Ascites

Isotype: IgG1 kappa

Clone: DF3

Specificity: This antibody is specific to 300 kD DF3 antigen present on the apical borders of secretory mammary epithelial cells. It stains glandular epithelia of breast, colon, kidney, liver, lung, pancreas, salivary gland and skin. In tumor tissues, it stains carcinomas of the breast, gastrointestinal tract, lung, kidney, urinary bladder and ovary.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union