CD5 Molecule (CD5) antibody
| Antigen |
|
| Synonyms |
T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790 |
| Clonality |
Monoclonal (4C7) |
|
Host
|
|
|
Reactivity
|
Human (181), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
|
|
Application
|
 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (294), Immunohistochemistry (Frozen Sections) (IHC (fro)) (77), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN125181 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
310.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
CD5
|
|
Immunogen
|
Recombinant prokaryotic fusion protein corresponding to the external domain of the CD5 molecule.
|
|
Format
|
Supernatant
|
|
Isotype
|
IgG1 kappa (Matching secondary antibodies)
|
|
Clone
|
4C7
|
|
Specificity
|
This antibody is specific to CD5 antigen. It stains a cell surface glycoprotein of 67 kD present on 95% of thymocytes and 72% of peripheral blood lymphocytes. The CD5 is expressed by many T cell leukemias, lymphomas and activated T cells. This antibody can be used for the detection of T cell acute lymphocytic leukemias.
|
|
Application Notes
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:20 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
|
|
Concentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
CD Antigens
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
Alternatives for antigen "CD5 Molecule (CD5)", type "Antibodies"
|
Hosts
|
Mouse (263), Rat (55), Rabbit (49)
|
|
Reactivities
|
Human (181), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
|
|
Applications
|
Flow Cytometry (FACS) (294), Immunohistochemistry (Frozen Sections) (IHC (fro)) (77), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
FITC (65), Biotin (43), PE (42), APC (15), RPE (10), Cy5.5 (3), Cy7,PE (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1), SPRD (1)
|
