CD68 (CD68) antibody
| Antigen |
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| Synonyms |
GP110, SCARD1, DKFZp686M18236 |
| Clonality |
Monoclonal (KP1) |
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Host
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Reactivity
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunofluorescence (IF) (29), Western Blotting (WB) (28), Immunoprecipitation (IP) (17), ELISA (4), Immunohistochemistry (IHC) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125194 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
185.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD68
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Immunogen
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Subcellular fraction of human alveolar macrophages.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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KP1
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Specificity
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This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:20-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD68 (CD68)", type "Antibodies"
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Hosts
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Mouse (69), Rabbit (33), Rat (23)
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Reactivities
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Human (72), Mouse (Murine) (43), Rat (Rattus) (34), Cow (Bovine) (7), Monkey (2), Cat (Feline) (1), Dog (Canine) (1), Guinea Pig (1), Pig (Porcine) (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Flow Cytometry (FACS) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunofluorescence (IF) (29), Western Blotting (WB) (28), Immunoprecipitation (IP) (17), ELISA (4), Immunohistochemistry (IHC) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (16), Biotin (8), PE (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Epitopes
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AA 155-355 (3), AA 1-164 (1)
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