CD75 (CD75) antibody

Antigen: CD75 (CD75)

Synonyms: ST6N, SIAT1, ST6GalI, MGC48859, Siat1, ST6GAL1, siat1, MGC136454, zgc:136454, DKFZp459I191

Clonality: Monoclonal (LN1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125196

Additional Information

Alternative name: CD75

Immunogen: Pokeweed mitogen stimulated peripheral mononuclear cells.

Format: Supernatant

Isotype: IgM kappa  (Matching secondary antibodies)

Clone: LN1

Specificity: This antibody recognizes CDw75 protein present on cell membrane and cytoplasm of germinal center B cells and B cell lymphomas. It does not react with T cells or T cell lymphomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CD75, B cell antibody