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Cytokeratin 18 (KRT18) antibody

Antigen

Cytokeratin 18 (KRT18)
Synonyms K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18
Clonality Monoclonal (DC10)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125206
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 206.00 $   Plus shipping costs $35.00
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anti-Cytokeratin 18 (KRT18) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name Cytokeratin 18
Immunogen BALB/C mice were injected with PMC-42 human breast carcinoma cell line.
Format Supernatant
Isotype IgG1  (Matching secondary antibodies)
Clone DC10
Specificity This antibody is specific to human cytokeratin 18 of 45 kD. Cytokeratin 18 belongs to a family of acidic type A and exists along with cytokeratin 8 in most simple ductal and glandular epithelia. This antibody does not reacts with squamous epithelium. It reacts with benign and malignant epithelial lesions as well as majority of adenocarcinomas and basal cell carcinomas.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Extracellular Matrix
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
Hosts Rabbit (75), Mouse (70), Guinea Pig (1)
Reactivities Human (124), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (9), Hamster (5), None (4), Sheep (Ovine) (3), Cat (Feline) (2), Bird (Avian) (1), Monkey (1)
Applications Western Blotting (WB) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (64), Flow Cytometry (FACS) (63), Immunofluorescence (IF) (58), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), ELISA (20), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (14), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
Conjugates FITC (13), Biotin (11), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
Epitopes pSer33 (7), C-Term (5), Center (1), N-Term (1)