Protein tyrosine Phosphatase, Receptor Type, C-Associated Protein (PTPRCAP) antibody

Antigen: Protein tyrosine Phosphatase, Receptor Type, C-Associated Protein (PTPRCAP)

Clonality: Monoclonal (17A5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125213

Additional Information

Alternative name: Lymphocyte Phosphatase-Associated Phosphoprotein (LPAP)

Immunogen: Prokaryotic recombinant protein corresponding to the entire cytoplasmic domain of the LPAP molecule.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 17A5

Specificity: Lymphocyte phosphatase-associate Phosphoprotein (LPAP) is a transmembrane phosphoprotein of 32 kD, which is only stable when expressed in the presence of the CD45 glycoprotein. It is expressed on both resting and activated T and B lymphocyte, NK cells and thymocytes. Terminally differentiated plasma cells, granulocytes and monocytes do not express LPAP.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Kinases/Phosphatases

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Schraven, Schoenhaut, Bruyns et al.: "LPAP, a novel 32-kDa phosphoprotein that interacts with CD45 in human lymphocytes." in: The Journal of biological chemistry, Vol. 269, Issue 46, pp. 29102-11, 1994 (PubMed).

Ding, Bruyns, Li et al.: "Biochemical and functional analysis of mice deficient in expression of the CD45-associated phosphoprotein LPAP." in: European journal of immunology, Vol. 29, Issue 12, pp. 3956-61, 2000 (PubMed).