CD7 (CD7) antibody
| Antigen |
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| Synonyms |
GP40, TP41, Tp40, LEU-9 |
| Clonality |
Monoclonal (CBC-37) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (110), Western Blotting (WB) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (24), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunoprecipitation (IP) (18), ELISA (5), Functional Studies (Func) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (IHC) (4), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125217 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
599.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD7
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Immunogen
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CEM T-cell line (ATCC CCL-199), a T lymphoblastoid cell line, established from a patient with acute lymphoblastic leukemia.
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Format
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Supernatant
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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CBC-37
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Specificity
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CD7 is a 40kD transmembrane, single-chain glycoprotein. CD7 is expressed by majority of peripheral blood T cells, NK cells and all Thymocytes. The presence or absence of CD7 is a useful marker for the classification of T- and NK-cell malignancies. The CD7 molecule is absent in a subset of human T cells under certain physiologic conditions. Thus an increase in CD7 negative T cells can be seen in some inflammatory skin disease.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD7 (CD7)", type "Antibodies"
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Hosts
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Mouse (109), Rabbit (43), Rat (4)
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Reactivities
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Human (153), Mouse (Murine) (20), Rat (Rattus) (20), Cow (Bovine) (5), Horse (Equine) (5), Pig (Porcine) (5), Rabbit (5), Dog (Canine) (4), Chicken (2), Sheep (Ovine) (2)
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Applications
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Flow Cytometry (FACS) (110), Western Blotting (WB) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (24), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunoprecipitation (IP) (18), ELISA (5), Functional Studies (Func) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (IHC) (4), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (17), Biotin (16), PE (13), APC (6), Cy5,PE (4), Alexa Fluor 488 (2), RPE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy5.5,PE (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), PerCP (1), RBITC (1), RPE,Cy5 (1)
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Alternatives 
