MUM1 Protein antibody

Antigen: MUM1 Protein

Clonality: Monoclonal (MUM1p)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125218

Additional Information

Immunogen: Recombinant GST-MUM1 protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: MUM1p

Specificity: Multiple myeloma oncogene-1 (MUM1) is a 50kD protein encoded by the MUM1 gene. This antibody stains MUM1 protein, which is expressed in a subset of B cells in the light zone of the germinal center, plasma cells, activated T cells and a wide spectrum of related hematolymphoid neoplasms. This antibody is useful in the sub classification of lymphoid malignancies.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 1 mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear and cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Gaidano, Carbone: "MUM1: a step ahead toward the understanding of lymphoma histogenesis." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 14, Issue 4, pp. 563-6, 2000 (PubMed).

Natkunam, Warnke, Montgomery et al.: "Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 14, Issue 7, pp. 686-94, 2001 (PubMed).