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CD3 (CD3E) antibody

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (F7-2-38)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC)
Catalog no. ABIN125220
Quantity 1ml  (100ug/ml approximately)  (Variants)
Price 526.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD3
Immunogen Purified CD3 epsilon gamma delta/CD3 omega
Format Supernatant
Isotype IgG1  (Matching secondary antibodies)
Clone F7-2-38
Specificity CD3 is first detectable in early Thymocytes and its appearance probably represents one of the earliest signs of commitment to T cell lineage. In cortical Thymocytes, the antigen is predominantly present as an intracytoplasmic constituent. This antibody labels CD3 epsilon and is useful tool for the identification of T cells and related neoplasms.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 1 mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane and cytoplasmic
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (302), Rat (53), Rabbit (49), Hamster (35)
Reactivities Human (284), Mouse (Murine) (100), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (18), Cow (Bovine) (17), Monkey (15), Primate (10), Bird (Avian) (4), Cat (Feline) (4), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (347), Immunohistochemistry (Frozen Sections) (IHC (fro)) (86), Immunoprecipitation (IP) (61), Functional Studies (Func) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Western Blotting (WB) (36), Immunofluorescence (IF) (31), ELISA (19), Immunohistochemistry (IHC) (16), Blocking Antibody (Inhibition) (10), Immunocytochemistry (ICC) (6), Enzyme Immunoassay (EIA) (3), Cytotoxicity Test (CyTox) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (46), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)