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O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody

Antigen

O6-Methylguanine-DNA-Methyltransferase (MGMT)
Synonyms MGMT
Clonality Monoclonal (MT3-1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN125221
Quantity 1ml  (100ug/ml approximately)  (Variants)
Price 526.00 $   Plus shipping costs $35.00
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Additional Information
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Immunogen Recombinant human MGMT protein.
Format Supernatant
Isotype IgG1  (Matching secondary antibodies)
Clone MT3-1
Specificity O6-Methylguanine-DNA-Methyltransferase (MGMT) is a 22 kD human DNA repair protein that removes O6-alkylguanine DNA adducts. MGMT acts as a suicide acceptor protein repairing the DNA by accepting alkyl groups and consequently inactivating itself. This antibody stains all mantle zone lymphocytes and 50%of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear and cytoplasmic
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Secondary Antibodies Variants back to top

Publications Egyhuazi, Hansson, Ringborg: "O6-methylguanine-DNA methyltransferase activities in biopsies of human melanoma tumours." in: British journal of cancer, Vol. 71, Issue 1, pp. 37-9, 1995 (PubMed).

Matsukura, Miyazaki, Yakushiji et al.: "Expression and prognostic significance of O6-methylguanine-DNA methyltransferase in hepatocellular, gastric, and breast cancers." in: Annals of surgical oncology, Vol. 8, Issue 10, pp. 807-16, 2002 (PubMed).

Gerson: "Clinical relevance of MGMT in the treatment of cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 20, Issue 9, pp. 2388-99, 2002 (PubMed).

Alternatives
Application Details Publications Secondary Antibodies Variants back to top

Alternatives for antigen "O6-Methylguanine-DNA-Methyltransferase (MGMT)", type "Antibodies"
Hosts Rabbit (38), Mouse (28)
Reactivities Human (62), Mouse (Murine) (22), Rat (Rattus) (22), Hamster (1)
Applications Western Blotting (WB) (41), Flow Cytometry (FACS) (34), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunoprecipitation (IP) (8), ELISA (6), Immunohistochemistry (IHC) (6), ELISA (Detection) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (1)