Hepatocyte Specific Antigen antibody

Antigen: Hepatocyte Specific Antigen

Clonality: Monoclonal (OCH1E5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125223

Additional Information

Immunogen: Human liver fixed in formalin.

Format: Supernatant

Isotype: IgG1

Clone: OCH1E5

Specificity: Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes. This antibody recognizes an uncharacterized antigen present in both adults and fetal normal hepatocytes to produce a distinct granular cytoplasmic staining. This antibody stains majority of hepatocellular carcinomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed tissues do not require any special treatment. Positive Control: Liver. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human liver stained with Hepatocyte antibody

Publications

Publications: Schleger, Heck, Niketeghad et al.: "Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers." in: Experimental cell research, Vol. 236, Issue 2, pp. 418-26, 1997 (PubMed).

Fasano, Theise, Nalesnik et al.: "Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 11, Issue 10, pp. 934-8, 1998 (PubMed).