MutS Homolog 6 (E. Coli) (MSH6) antibody

Antigen: MutS Homolog 6 (E. Coli) (MSH6)

Synonyms: GTBP, HSAP, HNPCC5, cb326, wu:fb36c12, MSH6, LOC100227810

Clonality: Monoclonal (44)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125226

Additional Information

Alternative name: MSH6

Immunogen: Synthetic human MSH6 peptide.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: 44

Specificity: MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. This antibody cross reacts with mouse, rat and dog.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil, colon carcinoma. Cellular localization: Nuclear Other Applications: immunoprecipitation and Western blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as affinity purified immunoglobulin liquid containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon carcinoma stained with MSH6 antibody

Publications

Publications: Schleger, Heck, Niketeghad et al.: "Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers." in: Experimental cell research, Vol. 236, Issue 2, pp. 418-26, 1997 (PubMed).

Fasano, Theise, Nalesnik et al.: "Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 11, Issue 10, pp. 934-8, 1998 (PubMed).

Bressenot, Marcon, Montagne et al.: "Clear cell adenocarcinoma: a rare variant of primary colonic tumour." in: International journal of colorectal disease, Vol. 23, Issue 1, pp. 137-8, 2007 (PubMed).