Caudal Type Homeobox 2 (CDX2) antibody

Antigen: Caudal Type Homeobox 2 (CDX2)

Synonyms: CDX3, CDX-3, CDX2, CDX-2, MGC130987, cdx3-like

Clonality: Monoclonal (AMT28)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125229

Additional Information

Alternative name: CDX2

Immunogen: Prokaryotic recombinant protein corresponding to the 180 amino acid N-terminal region of the human CDX2 molecule.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: AMT28

Specificity: CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Colon cancer. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as liquid culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Caudal Type Homeobox 2 (CDX2) antibody

Publications

Publications: Silberg, Swain, Suh et al.: "Cdx1 and cdx2 expression during intestinal development." in: Gastroenterology, Vol. 119, Issue 4, pp. 961-71, 2000 (PubMed).

Hinoi, Tani, Lucas et al.: "Loss of CDX2 expression and microsatellite instability are prominent features of large cell minimally differentiated carcinomas of the colon." in: The American journal of pathology, Vol. 159, Issue 6, pp. 2239-48, 2001 (PubMed).

Bai, Yamamoto, Akiyama et al.: "Ectopic expression of homeodomain protein CDX2 in intestinal metaplasia and carcinomas of the stomach." in: Cancer letters, Vol. 176, Issue 1, pp. 47-55, 2002 (PubMed).

Terada: "Intraductal tubular carcinoma, intestinal type, of the pancreas." in: Pathology international, Vol. 59, Issue 1, pp. 53-8, 2009 (PubMed).