alpha-Methylacyl-CoA Racemase (AMACR) antibody

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyms: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Clonality: Monoclonal (13H4)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125233

Additional Information

Alternative name: P504S (AMACR)

Immunogen: Full length recombinant P504S.

Isotype: IgG

Clone: 13H4

Specificity: This antibody recognizes a 382 amino acid protein and a 54 kD protein. P504S has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 9.0 prior to immunostaining. Positive Control: Prostate adenocarcinoma, HEK293 cells. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing BSA and sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Xu, Stolk, Zhang et al.: "Identification of differentially expressed genes in human prostate cancer using subtraction and microarray." in: Cancer research, Vol. 60, Issue 6, pp. 1677-82, 2000 (PubMed).

Zhou, Jiang, Epstein: "Expression and diagnostic utility of alpha-methylacyl-CoA-racemase (P504S) in foamy gland and pseudohyperplastic prostate cancer." in: The American journal of surgical pathology, Vol. 27, Issue 6, pp. 772-8, 2003 (PubMed).

Jiang, Fanger, Woda et al.: "Expression of alpha-methylacyl-CoA racemase (P504s) in various malignant neoplasms and normal tissues: astudy of 761 cases." in: Human pathology, Vol. 34, Issue 8, pp. 792-6, 2003 (PubMed).