Solute Carrier Family 45, Member 3 (SLC45A3) antibody

Antigen: Solute Carrier Family 45, Member 3 (SLC45A3)

Synonyms: RGD1309764, MGC68967, SLC45A3, zgc:162897, PRST, IPCA6, IPCA-2, IPCA-6, IPCA-8, PCANAP2, PCANAP6, PCANAP8, Pcanap6, AU023994, AU043764, MGC32471, Prostein, 2210413P12Rik

Clonality: Monoclonal (10E3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125234

Additional Information

Alternative name: P501S (Prostein)

Immunogen: BALB/C mice were injected with recombinant truncated prostein containing amino acids 341-527.

Format: Ig-fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 10E3

Specificity: This antibody recognizes a 553 amino acid protein and reacts with a 21 kD recombinant prostein fragment and with a 50 kD doublet. This antibody has been tested on a broad array of neoplastic tissues and found to be unreactive with all non-prostate malignant tissues. It stains majority of primary and metastatic prostate carcinomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing BSA and sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Xu, Kalos, Stolk et al.: "Identification and characterization of prostein, a novel prostate-specific protein." in: Cancer research, Vol. 61, Issue 4, pp. 1563-8, 2001 (PubMed).

Kalos, Askaa, Hylander et al.: "Prostein expression is highly restricted to normal and malignant prostate tissues." in: The Prostate, Vol. 60, Issue 3, pp. 246-56, 2004 (PubMed).