Prostate Specific Membrane Antigen (PSMA) antibody

Antigen: Prostate Specific Membrane Antigen (PSMA)

Clonality: Monoclonal (3-00E-006)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125235

Additional Information

Immunogen: BALB/C mice were injected with LNCaP membrane preparation and Immunoaffinity purified PSMA.

Isotype: IgG1

Clone: 3-00E-006

Specificity: Prostate specific membrane antigen (PSMA) is a 750 amino acid type II membrane glycoprotein. PSMA is expressed in normal and malignant prostatic epithelium and in a subset of non-prostatic tissues. PSMA expression has been shown to correlate with the progression of prostate cancer, with highest levels expressed in hormone-refractory and metastatic disease. PSMA expresses in neovasculature of a variety of non-prostatic solid tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 9.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic and/or membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing BSA and sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell/Tissue Markers, Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Silver, Pellicer, Fair et al.: "Prostate-specific membrane antigen expression in normal and malignant human tissues." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 3, Issue 1, pp. 81-5, 1999 (PubMed).

Chang, Reuter, Heston et al.: "Five different anti-prostate-specific membrane antigen (PSMA) antibodies confirm PSMA expression in tumor-associated neovasculature." in: Cancer research, Vol. 59, Issue 13, pp. 3192-8, 1999 (PubMed).