B-Cell CLL/lymphoma 10 (BCL10) antibody

Antigen: B-Cell CLL/lymphoma 10 (BCL10)

Synonyms: CLAP, mE10, CIPER, c-E10, CARMEN, ME10, cE10, BCL-10, C81403, AI132454, BCL10, MGC108338

Clonality: Monoclonal (151)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125237

Additional Information

Alternative name: Bcl-10

Immunogen: Human recombinant Bcl-10

Isotype: IgG1  (Matching secondary antibodies)

Clone: 151

Specificity: Monoclonal Anti-Bcl-10 reacts specifically with human Bcl-10. The epitope recognized by the antibody resides within amino acids 122-168 of human Bcl-10 molecule. Bcl-10, also designated as CIPER, mE10, cE10, CARMEN, and CLAP, is an N-terminal CARD (Caspase Recruitment Domain) containing protein. It is a cellular homologue of the equine herpesvirus-2 protein E-10 (vCLAP). Bcl-10 was implicated in the regulation of apoptosis by interacting with caspase 9, enhancing procaspase 9 processing, and triggering its activation when overexpressed in the cell. Bcl-10 cellular overexpression induces JNK, p38, and NF-kB activation. Deregulation of Bcl-10 expression was also demonstrated to be involved in cellular oncogenesis.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin by protein G chromatography containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Willis, Jadayel, Du et al.: "Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types." in: Cell, Vol. 96, Issue 1, pp. 35-45, 1999 (PubMed).

Srinivasula, Ahmad, Lin et al.: "CLAP, a novel caspase recruitment domain-containing protein in the tumor necrosis factor receptor pathway, regulates NF-kappaB activation and apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17946-54, 1999 (PubMed).

Du, Peng, Liu et al.: "BCL10 gene mutation in lymphoma." in: Blood, Vol. 95, Issue 12, pp. 3885-90, 2000 (PubMed).