Cytokeratin 1 (KRT1) antibody
| Antigen |
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| Synonyms |
K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK |
| Clonality |
Monoclonal (C-11) |
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Host
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Reactivity
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Human (30), Rat (Rattus) (6), Chicken (4), Cow (Bovine) (4), Mouse (Murine) (3), Rabbit (3), Frog (2), Mammalian (2), Snake (2), Dog (Canine) (1), Hamster (1), Horse (Equine) (1), Monkey (1), Pig (Porcine) (1), Salmonella typhi (S. typhi) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125239 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
219.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin
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Immunogen
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Keratin enriched preparation from cultured human epidermoid carcinoma cell line A431.
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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C-11
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Specificity
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Monoclonal Anti-Pan Cytokeratin (clone C-11) recognizes human cytokeratins 4, 5, 6, 8, 10, 13 and 18 in immunoblotting. It is a broad-spectrum antibody, which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody cross reacts with a wide interspecies cross-reactivity e.g., human, bovine, rat, frog.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as diluted ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Kasper: "Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities." in: Histochemistry, Vol. 95, Issue 6, pp. 613-20, 1991 (PubMed).
Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).
Buartkovua, Buartek, Lukuas et al.: "Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology." in: Neoplasma, Vol. 38, Issue 4, pp. 439-46, 1991 (PubMed).
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Alternatives for antigen "Cytokeratin 1 (KRT1)", type "Antibodies"
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Hosts
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Mouse (32), Guinea Pig (2), Rabbit (2)
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Reactivities
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Human (30), Rat (Rattus) (6), Chicken (4), Cow (Bovine) (4), Mouse (Murine) (3), Rabbit (3), Frog (2), Mammalian (2), Snake (2), Dog (Canine) (1), Hamster (1), Horse (Equine) (1), Monkey (1), Pig (Porcine) (1), Salmonella typhi (S. typhi) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Western Blotting (WB) (10), Immunohistochemistry (IHC) (6), Flow Cytometry (FACS) (3), Immunofluorescence (IF) (3), Enzyme Immunoassay (EIA) (2), ELISA (1), Immunocytochemistry (ICC) (1)
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Conjugates
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APC (1), PE (1)
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