Cytokeratin 7 (KRT7) antibody

Antigen: Cytokeratin 7 (KRT7)

Synonyms: KRT7, CK7, K7, Krt2-7, MGC11625, D15Wsu77e, SCL, K2C7, MGC3625, MGC129731

Clonality: Monoclonal (LDS-68)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125243

Additional Information

Alternative name: Cytokeratin 7

Immunogen: BALB/C mice were immunized with human bladder carcinoma cell line, RT4.

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: LDS-68

Specificity: This antibody recognizes keratin polypeptide of 54 kD. This antibody reacts specifically with most simple epithelial cells but shows no reaction with epithelial cells in the intestine or stomach. Cholangiocellular carcinoma, ductal carcinoma of breast and transitional carcinoma of urinary bladder are positive but colon rectal adenocarcinoma and hepatocellular carcinoma does not show any staining.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

van Niekerk, Jap, Ramaekers et al.: "Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues." in: The Journal of pathology, Vol. 165, Issue 2, pp. 145-52, 1992 (PubMed).

Poels, Jap, Ramaekers et al.: "Characterization of a hormone-producing ovarian carcinoma cell line." in: Gynecologic oncology, Vol. 32, Issue 2, pp. 203-14, 1989 (PubMed).