P63 antibody

Antigen: P63

Clonality: Monoclonal (Y4A3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125245

Additional Information

Immunogen: BALB/C mice were immunized with a recombinant protein from the N-terminus.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: Y4A3

Specificity: This antibody recognizes a 63 kD protein. The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: colon ca. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Signaling, Apoptosis/Necrosis

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human prostate stained with Cytokeratin 5/14 antibody

Publications

Publications: Yang, Kaghad, Wang et al.: "p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities." in: Molecular cell, Vol. 2, Issue 3, pp. 305-16, 1998 (PubMed).

Yang, Schweitzer, Sun et al.: "p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development." in: Nature, Vol. 398, Issue 6729, pp. 714-8, 1999 (PubMed).