Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Clonality: Monoclonal (DCS-72)

Host: Mouse

Reactivity: Human, Monkey, Dog (Canine), Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125247

Additional Information

Alternative name: p27KIP1

Immunogen: BALB/C mice were injected with mouse recombinant p27KIP1 protein.

Isotype: IgG1  (Matching secondary antibodies)

Clone: DCS-72

Specificity: Monoclonal Anti-p27Kip1 recognizes p27Kip1 using immunoblotting (27 kDa), immunocytochemistry and immunoprecipitation. Cross- reactivity has been observed with human, monkey, dog, rat and mousep27Kip1. Higher and lower molecular weight bands, which may represent a polyubiquinated species and breakdown products of p27Kip1, respectively, may also be detected in some preparations, using immunoblotting.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted mouse ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union