Cyclin-Dependent Kinase Inhibitor 2C (p18, Inhibits CDK4) (CDKN2C) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 2C (p18, Inhibits CDK4) (CDKN2C)

Synonyms: p18, INK4C, p18-INK4C, INK4c, INK4d, C77269, p18INK4c, CDKN2C, ink4c, p18-ink4c

Clonality: Monoclonal (DCS-118)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125249

Additional Information

Alternative name: p18INK4C

Immunogen: BALB/C mice were injected with recombinant human p18INK4C protein.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: DCS-118

Specificity: This antibody is specific to a human p18INK4C. The product does not show cross-reaction with the closely related CDK inhibitors, such as p15INK4b, p16INK4a and p19INK4d. The four known 15- to 19 kD INK4 proteins (p15INK4b, p16INK4a, p18INK4c and p19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs. p18INK4C is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted mouse ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union