Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody

Antigen: Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1)

Synonyms: flt1b, VEGFR1, FLT, Flt-1, sFlt1, VEGFR-1, AI323757, FLT1

Clonality: Monoclonal (FLT-11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125252

Additional Information

Alternative name: Vascular Endothelial Growth Factor Receptor-1 (Flt-1)

Immunogen: Human recombinant extracellular domain of VEGF receptor-1.

Isotype: IgG1

Clone: FLT-11

Specificity: Monoclonal Anti- VEGF Receptor-1 (Flt1 Receptor) reacts specifically with the extracellular domain of human VEGF Receptor-1 (Flt1 Receptor). The epitope recognized by the antibody resides within amino acid residues 1-251 of the VEGF Receptor-1 molecule. This antibody does not recognize VEGF Receptor-2 (KDR). It cross reacts with human and mouse.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Angiosarcoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 28o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Sait, Dougher-Vermazen, Shows et al.: "The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-->q12." in: Cytogenetics and cell genetics, Vol. 70, Issue 1-2, pp. 145-6, 1995 (PubMed).

Waltenberger, Claesson-Welsh, Siegbahn et al.: "Different signal transduction properties of KDR and Flt1, two receptors for vascular endothelial growth factor." in: The Journal of biological chemistry, Vol. 269, Issue 43, pp. 26988-95, 1994 (PubMed).

Hanahan: "Signaling vascular morphogenesis and maintenance." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 48-50, 1997 (PubMed).