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Tumor Necrosis Factor (TNF) antibody
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|3 references available|
|Price||488.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Alternative name||Tumor Necrosis Factor alpha (TNF alpha)|
|Immunogen||Purified recombinant human tumor necrosis factor-alpha.|
|Specificity||Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is a member of the TNF superfamily of cytokines. TNF-alpha is expressed as a 26 kD membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kD monomer, which forms homotrimers in circulation. The antibody does not cross-react with recombinant human TNF-alpha, TNF sRI, TNF sRII, nor with recombinant mouse TNF-alpha.|
|Synonyms||DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin|
|Application Notes||Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Spleen. Cellular localization: Cell membrane, Extracellular|
|Buffer||This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.|
|Storage||Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation|
|Restrictions||For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union|
Beutler, Cerami: "The history, properties, and biological effects of cachectin." in: Biochemistry, Vol. 27, Issue 20, pp. 7575-82, 1989 (PubMed).
Broudy, Kaushansky, Segal et al.: "Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7467-71, 1986 (PubMed).
Balosso, Ravizza, Perego et al.: "Tumor necrosis factor-alpha inhibits seizures in mice via p75 receptors." in: Annals of neurology, Vol. 57, Issue 6, pp. 804-12, 2005 (PubMed).