Nerve Growth Factor Receptor (NGFRp75) antibody

Antigen: Nerve Growth Factor Receptor (NGFRp75)

Clonality: Monoclonal (ME20-4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125254

Additional Information

Immunogen: Human melanoma cell line WM245.

Isotype: IgG1  (Matching secondary antibodies)

Clone: ME20-4

Specificity: Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kD), also known as gp75NGFR, p75NGFR and p75NTR. The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon, but not with rat, chicken or mouse NGFR.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cell surface, cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Growth Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Suburo, Gu, Moscoso et al.: "Developmental pattern and distribution of nerve growth factor low-affinity receptor immunoreactivity in human spinal cord and dorsal root ganglia: comparison with synaptophysin, neurofilament and neuropeptide immunoreactivities." in: Neuroscience, Vol. 50, Issue 2, pp. 467-82, 1992 (PubMed).

Caneva, Soligo, Cattoretti et al.: "Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies." in: Blood cells, molecules & diseases, Vol. 21, Issue 2, pp. 73-85, 1996 (PubMed).

Ibuaunez: "Emerging themes in structural biology of neurotrophic factors." in: Trends in neurosciences, Vol. 21, Issue 10, pp. 438-44, 1998 (PubMed).