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Epidermal Growth Factor Receptor (EGFR) antibody

Antigen

Epidermal Growth Factor Receptor (EGFR)
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33,  ... show more
Clonality Monoclonal (111-6)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Catalog no. ABIN125258
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 256.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Immunogen BALB/C mice were injected with extracellular domain of human recombinant EGFR protein.
Format Liquid (prediluted)
Isotype IgG1  (Matching secondary antibodies)
Clone 111-6
Specificity This antibody is specific to 170 kD known as first member (EGF- Receptor) of type I Family of growth factor receptors. Its epitope maps in the extracellular domain of EGFR. Overexpression of EGFR has been reported in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. EGFR is over expressed in squamous cell carcinoma.
Synonyms ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature or overnight at 40C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 370C. Positive Control: Placenta. Cellular localization: Cell membrane Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts Rabbit (392), Mouse (165), Rat (8), Sheep (7), Goat (2)
Reactivities Human (555), Mouse (Murine) (263), Rat (Rattus) (238), Dog (Canine) (33), Rabbit (18), Pig (Porcine) (17), Cow (Bovine) (6), Chicken (5), Horse (Equine) (2), Sheep (Ovine) (2)
Applications Western Blotting (WB) (400), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (131), Immunoprecipitation (IP) (119), Enzyme Immunoassay (EIA) (103), Immunohistochemistry (IHC) (103), Immunofluorescence (IF) (95), ELISA (92), Flow Cytometry (FACS) (90), Immunohistochemistry (Frozen Sections) (IHC (fro)) (40), Blocking Antibody (Inhibition) (16), Dot Blot (Dot) (14), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2), Blocking Antibody (Inhibition) (1)
Conjugates Biotin (35), FITC (5), Alexa Fluor 488 (3), PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes pTyr1197 (17), C-Term (16), pTyr869 (13), pTyr1092 (12), pTyr1110 (11), pTyr1172 (11), pThr678 (10), pSer1070 (9), pThr693 (9), N-Term (5), pTyr1016 (3), pTyr1069 (3), AA 1-203 (2), AA 1140-1160 (2), AA 960-980 (2), Extracell. non Ligand binding Site (2), S695 (2), Y1125 (2), Y869 (2), Y998 (2), pSer1071 (2), pTyr1173 (2), AA 1189-1199 (1), AA 985-996 (1), Ab-1016 (1), Ab-1026 (1), Ab-1069 (1), Ab-1071 (1), Amino Acids 1165-1186 (1), External Domain (1), Extracellular non-ligand binding site (1), S1026 (1), S1070 (1), S1071 (1), S1166 (1), S1190 (1), S991 (1), T678 (1), TYR1173 (1), Y1016 (1), Y1069 (1), Y1092 (1), Y1110 (1), Y1172 (1), Y978 (1), extracellular ligand binding site (1), internal (1), pS1070 (1), pS768 (1), pSer1026 (1), pSer1166 (1), pSer1190 (1), pSer695 (1), pSer768 (1), pTyr1125 (1), pTyr992 (1), pTyr998 (1), pY1016 (1), pY1092 (1), pY1172 (1), prylated At Threonine 654 (1), prylated At Tyrosine 1045 (1), prylated At Tyrosine 1173 (1), prylated At Tyrosine 845 (1), prylated at Serine 1047 (1)