Keratin 6 (KRT6) antibody
| Antigen |
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| Synonyms |
Krt2-6, Krt-2.6 |
| Clonality |
Monoclonal (LHK6B) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125261 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
135.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin 6
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Immunogen
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BALB/C mice were injected with a synthetic peptide (GSSTIKYTTTS) from the c- terminus of human cytokeratin 6.
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Format
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Liquid (prediluted)
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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LHK6B
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Specificity
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This antibody is specific to a 56 kD protein, which is identified as cytokeratin 6. Cytokeratin 6 and 16 are expressed in keratinocytes undergoing hyper proliferation. Cytokeratin is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia. Cytokeratin 6 is strongly expressed in about 75% of head and neck squamous cell carcinoma.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Wetzels, Kuijpers, Lane et al.: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).
Chamcheu, Loriue, Akgul et al.: "Characterization of immortalized human epidermolysis bullosa simplex (KRT5) cell lines: trimethylamine N-oxide protects the keratin cytoskeleton against disruptive stress condition." in: Journal of dermatological science, Vol. 53, Issue 3, pp. 198-206, 2009 (PubMed).
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Alternatives for antigen "Keratin 6 (KRT6)", type "Antibodies"
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Hosts
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Mouse (17), Rabbit (9), Guinea Pig (1)
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Reactivities
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Human (24), Mouse (Murine) (4), Rat (Rattus) (2), Zebrafish (Danio rerio) (2)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Western Blotting (WB) (11), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunohistochemistry (IHC) (7), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunoprecipitation (IP) (2), Immunofluorescence (IF) (1)
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Epitopes
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C-Term (8)
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