Estrogen Receptor (ER) antibody

Antigen: Estrogen Receptor (ER)

Synonyms: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Clonality: Monoclonal (SP1)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125264

Additional Information

Alternative name: Estrogen Receptor

Immunogen: Synthetic peptide derived from C-terminal of human estrogen receptor.

Format: Supernatant

Isotype: IgG  (Matching secondary antibodies)

Clone: SP1

Specificity: This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). ER content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This antibody crossreacts with Human, Mouse, Rat and Hamster.

Synonyms: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast Carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Estrogen Receptor (ER) antibody

Publications

Publications: Cano, Milanezi, Leituao et al.: "Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2." in: Diagnostic cytopathology, Vol. 29, Issue 4, pp. 207-11, 2003 (PubMed).