Cyclin D1 (CCND1) antibody

Antigen: Cyclin D1 (CCND1)

Synonyms: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Clonality: Monoclonal (SP4)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125266

Additional Information

Alternative name: Cyclin D1

Immunogen: A synthetic peptide from C- terminus of human cyclin D1.

Format: Supernatant

Isotype: IgG

Clone: SP4

Specificity: Cyclin D1 or PRAD-1 or bcl- 1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). This antibody crossreacts with Human, mouse and rat.

Synonyms: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast Carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western blotting (1:100-500).

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Cyclin D1 (CCND1) antibody

Publications

Cheuk, Wong, Wong et al.: "Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody." in: The American journal of surgical pathology, Vol. 28, Issue 6, pp. 801-7, 2004 (PubMed).

Klopstock, Katzenellenbogen, Pappo et al.: "HCV tumor promoting effect is dependent on host genetic background." in: PLoS ONE, Vol. 4, Issue 4, pp. e5025, 2009 (PubMed).