Ki-67 antibody
| Antigen |
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| Clonality |
Monoclonal (SP6) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125267 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
258.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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A synthetic peptide from C-terminus of human Ki-67.
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Format
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Supernatant
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP6
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Specificity
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Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. This antibody crossreacts with Human and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Proliferation Markers, Cell Cycle
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Ki-67", type "Antibodies"
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Hosts
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Rabbit (43), Mouse (18)
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Reactivities
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Human (57), Rat (Rattus) (23), Mouse (Murine) (14), Rabbit (6), Dog (Canine) (5), Horse (Equine) (5), Pig (Porcine) (5), Cow (Bovine) (3), Chicken (2)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Flow Cytometry (FACS) (16), Immunofluorescence (IF) (16), Immunohistochemistry (IHC) (14), Western Blotting (WB) (13), ELISA (7), Immunocytochemistry (ICC) (2), Enzyme Immunoassay (EIA) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2)
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Epitopes
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C-Term (8)
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