CD5 Molecule (CD5) antibody
| Antigen |
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| Synonyms |
T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790 |
| Clonality |
Monoclonal (SP19) |
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Host
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Reactivity
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Human (181), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (294), Immunohistochemistry (Frozen Sections) (IHC (fro)) (77), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125274 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD5
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Immunogen
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Synthetic peptide from the intracellular region of CD5.
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Format
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Supernatant
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP19
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Specificity
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This antibody recognizes CD5, which is a transmembrane protein, is found on 95%of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5.This antibody crossreacts with Human.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD5 Molecule (CD5)", type "Antibodies"
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Hosts
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Mouse (264), Rat (55), Rabbit (48)
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Reactivities
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Human (181), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
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Applications
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Flow Cytometry (FACS) (294), Immunohistochemistry (Frozen Sections) (IHC (fro)) (77), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (65), Biotin (43), PE (42), APC (15), RPE (10), Cy5.5 (3), Cy7,PE (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1), SPRD (1)
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