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CD8a Molecule (CD8A) antibody

Antigen

CD8a Molecule (CD8A)
Synonyms CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Clonality Monoclonal (SP16)
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125275
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 283.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD8
Immunogen Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule.
Format Supernatant
Isotype IgG  (Matching secondary antibodies)
Clone SP16
Specificity This antibody recognizes CD8. CD8 molecule consists of two chains, termed alpha and Beta chain, which are expressed as a disulphide-linked alpha/Beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/Beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not Beta chain).This antibody crossreacts with Human.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"
Hosts Mouse (300), Rat (103), Rabbit (76)
Reactivities Human (275), Mouse (Murine) (117), Rat (Rattus) (51), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Applications Flow Cytometry (FACS) (380), Immunohistochemistry (Frozen Sections) (IHC (fro)) (125), Immunoprecipitation (IP) (73), Immunofluorescence (IF) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Western Blotting (WB) (37), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (1)
Conjugates FITC (84), Biotin (47), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), PerCP (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitopes C-Term (1), N-Term (1)